Background and aim: Single-cell transcriptomics provides information on heterogeneity and common gene expression at the level of individual cells. This enables a cellular landscape to define intracellular gene regulatory networks. The aim of this study was to investigate mRNA expression of cMYC, OCT4, NANOG, SMAD2, and SMAD4 genes in embryos that are in the blastocyst stage.Materials and methods: We collected 150 embryos with high, medium and low-quality, and then were biopsied at the blastocyst stage. Extraction of total RNA from all embryos was performed and cDNA was synthesized. Expression of the target genes in mRNA levels were investigated by qReal-Time PCR technique.Result: Our results demonstrated that mRNA expression pattern of cMYC, OCT4, NANOG, SMAD2, and SMAD4 genes in low quality embryos is significantly different as compared high and medium quality embryos.Conclusion: In general, we suggested that mRNA expression pattern of developmental transcription factors may associated with embryos quality.